Abstract

Bacteria in the urine (Bacteriuria), if not properly managed by the host can turn from uncomplicated to complicated and thereby cause urinary tract infections. Ability of the urobacteria to produce biofilm may add to the already increase rate of antibiotic resistance thereby causing more challenges to health. The urobacteria from Olabisi Onabanjo Teaching Hospital were studied to know their ability to form biofilm and their reactions to some antibiotics. One hundred and sixty-eight mid-stream human urine produced by consented patients who visited the urology unit during the study were cultured on Cysteine lactose electrolyte-deficient agar and identified using microbiological standard methods. Biofilm test was done using the Congo red agar method, and Kirby-Bauer disc diffusion techniques were used to test their antibacterial reaction. One hundred and fifty-two identified pathogens were Escherichia coli (26.32%), Staphylococcus aureus (44.08%), Klebsiella pneumoniae (19.74%), Proteus mirabilis (4.61%), Pseudomonas aeruginosa (3.95%), and Candida albicans (1.32%). The highest occurrence was observed in the age group 31-40 years and among females. Biofilm formation was observed in 23 bacterial species isolated. The highest inhibitory zones observed against the biofilm-positive bacteria were augmentin (34 mm), ciprofloxacin (32 mm), and meropenem (30 mm) while total resistance was observed in K. pneumoniae. The findings revealed that females (young adults) are most affected by bacteriuria, with S. aureus being the most prevalent bacteria. Biofilm-positive bacteria were variably resistant to antibiotics tested, but the most sensitive ones like augmentin, ciprofloxacin, and meropenem can still be used for effective treatment in the study area.